| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Noecker, Elle A (11)
|
NITWST |
34.29 |
34.50 |
0.21 |
| 2 |
Brinker, Anna (12)
|
NITWST |
35.64 |
35.60 |
-0.04 |
| 3 |
Drozdowski, Jessica L (12)
|
NITWST |
38.69 |
38.84 |
0.15 |
| 4 |
Sauer, Emma L (12)
|
NIHAMB |
41.53 |
39.51 |
-2.02 |
| 5 |
Bembenista, Julia C (12)
|
NITWST |
41.23 |
41.35 |
0.12 |
| 6 |
Krumpek, Ella E (11)
|
NITWST |
47.87 |
43.31 |
-4.56 |
| 7 |
Moldenhauer, Paige A (11)
|
NIEAST |
41.79 |
43.54 |
1.75 |
| 8 |
Daley, Lily K (12)
|
NITWST |
43.42 |
43.82 |
0.40 |
| 9 |
Noecker, Sophia E (11)
|
NITWST |
44.97 |
47.83 |
2.86 |
| 10 |
Walters, Elizabeth J (11)
|
NITWST |
52.23 |
48.92 |
-3.31 |
| 11 |
Kerins, Bailey R (11)
|
NIHAMB |
53.48 |
49.80 |
-3.68 |
| 12 |
Mersinger, Sophia R (11)
|
NITWST |
|
50.12 |
- |
| 13 |
Pecorella, Paige A (11)
|
NIEMAC |
54.28 |
51.72 |
-2.56 |
| 14 |
Wilson, Gabrielle O (11)
|
NITWST |
56.36 |
55.15 |
-1.21 |
| 15 |
Rodgers, Morgan M (11)
|
NITWST |
|
1:03.84 |
- |
|
Killelea, Keira m (11)
|
NITTAC |
53.69 |
NS |
- |